In this study, a rapid separation of eleven polyphenols and three methylxanthines was obtained by means of nano-liquid chromatography (nano-LC), employing a 100μm I.D. capillary column packed with C18 core-shell particles (2.7μm) for 10cm. All compounds were baseline resolved with a step gradient elution in less than 15min. The developed analytical method was validated and the resulting RSD% for intra-day and inter-day repeatability, related to retention time, retention factor and peak area, were below 5.1 and 5.7%. LOD and LOQ values corresponded to 0.300 and 0.625μg/mL, while linearity range assessed gave R 2 no lower than 0.990. Then, the method was used to determine studied compounds in tea extracts. Further, the nano-LC system was coupled with a mass spectrometer to confirm the components present in real samples. Finally the results were compared with those obtained using a capillary column (100μm I.D.×10cm) packed with C18 sub-2μm particles, applying the same nano-LC experimental conditions. © 2012 Elsevier B.V.

Analysis of polyphenols and methylxantines in tea samples by means of nano-liquid chromatography utilizing capillary columns packed with core-shell particles

Fanali C;
2012-01-01

Abstract

In this study, a rapid separation of eleven polyphenols and three methylxanthines was obtained by means of nano-liquid chromatography (nano-LC), employing a 100μm I.D. capillary column packed with C18 core-shell particles (2.7μm) for 10cm. All compounds were baseline resolved with a step gradient elution in less than 15min. The developed analytical method was validated and the resulting RSD% for intra-day and inter-day repeatability, related to retention time, retention factor and peak area, were below 5.1 and 5.7%. LOD and LOQ values corresponded to 0.300 and 0.625μg/mL, while linearity range assessed gave R 2 no lower than 0.990. Then, the method was used to determine studied compounds in tea extracts. Further, the nano-LC system was coupled with a mass spectrometer to confirm the components present in real samples. Finally the results were compared with those obtained using a capillary column (100μm I.D.×10cm) packed with C18 sub-2μm particles, applying the same nano-LC experimental conditions. © 2012 Elsevier B.V.
2012
methylxanthines; polyphenols; nano-Liquid Chromatography; tea; mass spectrometry; core-shell stationary phase
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12610/6393
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