Objective: Identifying an objective, laboratory-based diagnostic tool (e.g., changes in gene expression), when used in conjunction with disease-specific clinical assessment, could increase the accuracy of the effectiveness of a therapeutic intervention. Methods: We assessed the association between treatment outcome and blood RNA expression before the therapeutic intervention to post-treatment (after one year) of five Autism Spectrum Disorder (ASD) toddlers who underwent an intensive cognitive-behavioral intervention integrated with psychomotor and speech therapy. Results: We found 113 significant Differentially Expressed genes (DEGs) enriched for the nervous system, immune system, and transcription and translation-related pathways. Some of these genes, as MALAT-1, TSPO, and CFL1, appear to be promising candidates. Conclusion: Our findings show that changes in peripheral gene expression could be used in conjunction with clinical scales to monitor a rehabilitation intervention's effectiveness in toddlers affected by ASD. These results need to be validated in a larger cohort.

Molecular biomarkers to track clinical improvement following an integrative treatment model in autistic toddlers

Sacco R.;Lintas C.
2021-01-01

Abstract

Objective: Identifying an objective, laboratory-based diagnostic tool (e.g., changes in gene expression), when used in conjunction with disease-specific clinical assessment, could increase the accuracy of the effectiveness of a therapeutic intervention. Methods: We assessed the association between treatment outcome and blood RNA expression before the therapeutic intervention to post-treatment (after one year) of five Autism Spectrum Disorder (ASD) toddlers who underwent an intensive cognitive-behavioral intervention integrated with psychomotor and speech therapy. Results: We found 113 significant Differentially Expressed genes (DEGs) enriched for the nervous system, immune system, and transcription and translation-related pathways. Some of these genes, as MALAT-1, TSPO, and CFL1, appear to be promising candidates. Conclusion: Our findings show that changes in peripheral gene expression could be used in conjunction with clinical scales to monitor a rehabilitation intervention's effectiveness in toddlers affected by ASD. These results need to be validated in a larger cohort.
2021
Autism Spectrum Disorder
Biomarkers
Differentially Expressed Genes (DEGs)
Integrative Treatment Model
Response to Therapy
Autism Spectrum Disorder
Biomarkers
Case-Control Studies
Child, Preschool
Cofilin 1
Cognitive Behavioral Therapy
Female
Gene Expression
Genome-Wide Association Study
Humans
Immune System
Integrative Medicine
Male
Nervous System
Protein Biosynthesis
RNA, Long Noncoding
Receptors, GABA
Transcription, Genetic
Treatment Outcome
Up-Regulation
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12610/64410
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