In the present study, the protective role of inulin against lipopolysaccharide (LPS)-induced oxidativestress was evaluated on human colonic mucosa using a proteomic approach. Humancolonic mucosa and submucosa were sealed between two chambers, with the luminal sidefacing upwards and overlaid with Krebs (control), LPS or LPS+ inulin IQ solution. The solutionson the submucosal side (undernatants) were collected following 30 min of mucosal exposure.iTRAQ based analysis was used to analyze the total soluble proteomes from humancolonic mucosa and submucosa treated with different undernatants. Human colonic musclestrips were exposed to the undernatants to evaluate the response to acetylcholine. Inulinexposure was able to counteract, in human colonic mucosa, the LPS-dependent alteration ofsome proteins involved in the intestinal contraction (myosin light chain kinase (MLCK), myosinregulatory subunit (MYL)), to reduce the up-regulation of two proteins involved in the radicalmediatedoxidative stress (the DNA-apurinic or apyrimidinic site) lyase) APEX1 and theT-complex protein 1 subunit eta (CCT7) and to entail a higher level of some detoxificationenzymes (the metallothionein-2 MT2A, the glutathione±S-transferase K GSTk, and two UDPglucuronosyltransferasesUGT2B4, UGT2B17). Inulin exposure was also able to prevent theLPS-dependent intestinal muscle strips contraction impairment and the mucosa glutathionelevel alterations. Exposure of colonic mucosa to inulin seems to prevent LPS-induced alterationin expression of some key proteins, which promote intestinal motility and inflammation,reducing the radical-mediated oxidative stress.

Effect of Inulin on Proteome Changes Induced by Pathogenic Lipopolysaccharide in Human Colon

Guarino MPL;Altomare A;Locato V;Alloni R;De Gara L;Cicala M
2017-01-01

Abstract

In the present study, the protective role of inulin against lipopolysaccharide (LPS)-induced oxidativestress was evaluated on human colonic mucosa using a proteomic approach. Humancolonic mucosa and submucosa were sealed between two chambers, with the luminal sidefacing upwards and overlaid with Krebs (control), LPS or LPS+ inulin IQ solution. The solutionson the submucosal side (undernatants) were collected following 30 min of mucosal exposure.iTRAQ based analysis was used to analyze the total soluble proteomes from humancolonic mucosa and submucosa treated with different undernatants. Human colonic musclestrips were exposed to the undernatants to evaluate the response to acetylcholine. Inulinexposure was able to counteract, in human colonic mucosa, the LPS-dependent alteration ofsome proteins involved in the intestinal contraction (myosin light chain kinase (MLCK), myosinregulatory subunit (MYL)), to reduce the up-regulation of two proteins involved in the radicalmediatedoxidative stress (the DNA-apurinic or apyrimidinic site) lyase) APEX1 and theT-complex protein 1 subunit eta (CCT7) and to entail a higher level of some detoxificationenzymes (the metallothionein-2 MT2A, the glutathione±S-transferase K GSTk, and two UDPglucuronosyltransferasesUGT2B4, UGT2B17). Inulin exposure was also able to prevent theLPS-dependent intestinal muscle strips contraction impairment and the mucosa glutathionelevel alterations. Exposure of colonic mucosa to inulin seems to prevent LPS-induced alterationin expression of some key proteins, which promote intestinal motility and inflammation,reducing the radical-mediated oxidative stress.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12610/7510
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 9
  • ???jsp.display-item.citation.isi??? ND
social impact