Objective: Type 1 diabetes and latent autoimmune diabetes in adults (LADA) are thought to result from immune-mediated beta-cell destruction. It remains unclear why LADA is clinically less severe compared to type 1 diabetes. This study aimed to compare the pro-inflammatory (interferon-gamma, IFN-gamma) and anti-inflammatory (interleukin-13, IL-13) T-cell responses in humans with LADA and type 1 diabetes. Research design and methods: IFN-gamma and IL-13 T-cell responses to a panel of 16 (auto)-antigens were tested using an enzyme linked immune-spot technique and peripheral T-cells from 35 patients with type 1 diabetes, 59 patients with type 2 diabetes, 23 LADA patients, and 42 control subjects. Results: LADA and type 1 diabetes patients did not display any statistically significant differences in the frequency of IFN-gamma or IL-13 responses to auto-antigenic stimuli, positive control or mitogen. Overall very low T cell reactivity to autoantigens was detected in all groups. IL-13 responses but not IFN-gamma responses to recall antigen tetanus toxoid were higher in healthy control subjects compared to patients with type 1 or type 2 diabetes or LADA (P< 0.05). Diabetes, independent of type, was associated with weaker response to recall antigen tetanus toxoid. Conclusions: LADA patients are indistinguishable from type 1 diabetes patients for cellular IFN-gamma and IL-13 responses upon mitogen and recall antigen stimulation. These results extend previous findings showing that systemic cytokine/chemokine and humoral responses in type 1 diabetes and LADA are similar. (C) 2012 Elsevier Ltd. All rights reserved.
Cellular interferon-gamma and interleukin-13 immune reactivity in type 1, type 2 and latent autoimmune diabetes: Action LADA 6
Pozzilli P;
2012-01-01
Abstract
Objective: Type 1 diabetes and latent autoimmune diabetes in adults (LADA) are thought to result from immune-mediated beta-cell destruction. It remains unclear why LADA is clinically less severe compared to type 1 diabetes. This study aimed to compare the pro-inflammatory (interferon-gamma, IFN-gamma) and anti-inflammatory (interleukin-13, IL-13) T-cell responses in humans with LADA and type 1 diabetes. Research design and methods: IFN-gamma and IL-13 T-cell responses to a panel of 16 (auto)-antigens were tested using an enzyme linked immune-spot technique and peripheral T-cells from 35 patients with type 1 diabetes, 59 patients with type 2 diabetes, 23 LADA patients, and 42 control subjects. Results: LADA and type 1 diabetes patients did not display any statistically significant differences in the frequency of IFN-gamma or IL-13 responses to auto-antigenic stimuli, positive control or mitogen. Overall very low T cell reactivity to autoantigens was detected in all groups. IL-13 responses but not IFN-gamma responses to recall antigen tetanus toxoid were higher in healthy control subjects compared to patients with type 1 or type 2 diabetes or LADA (P< 0.05). Diabetes, independent of type, was associated with weaker response to recall antigen tetanus toxoid. Conclusions: LADA patients are indistinguishable from type 1 diabetes patients for cellular IFN-gamma and IL-13 responses upon mitogen and recall antigen stimulation. These results extend previous findings showing that systemic cytokine/chemokine and humoral responses in type 1 diabetes and LADA are similar. (C) 2012 Elsevier Ltd. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
